[HTML][HTML] Construction and characterization of chimeric BHIV (BIV/HIV-1) viruses carrying the bovine immunodeficiency virus gag gene
YX Zhu, C Liu, XL Liu, WT Qiao, QM Chen… - World Journal of …, 2005 - ncbi.nlm.nih.gov
AIM: To explore the possibility of the replacement of the gag gene between human
immunodeficiency virus and bovine immunodeficiency virus, to achieve chimeric virions, and
thereby gain a new kind of AIDS vaccine based on BHIV chimeric viruses. METHODS: A
series of chimeric BHIV proviral DNAs differing in the replacement regions in gag gene were
constructed, and then were transfected into 293T cells. The expression of chimeric viral
genes was detected at the RNA and protein level. The supernatant of 293T cell was ultra …
immunodeficiency virus and bovine immunodeficiency virus, to achieve chimeric virions, and
thereby gain a new kind of AIDS vaccine based on BHIV chimeric viruses. METHODS: A
series of chimeric BHIV proviral DNAs differing in the replacement regions in gag gene were
constructed, and then were transfected into 293T cells. The expression of chimeric viral
genes was detected at the RNA and protein level. The supernatant of 293T cell was ultra …